Effect of different parameters on characteristics of doxorubicin loaded PCLF nanoparticles and evaluation of lymphatic uptake by in vivo imaging

摘要

In this study poly(caprolactone fumarate) (PCLF) copolymer has been used to prepare nanoparticles (NPs). Degradation of PCLF against time was evaluated in different mediums. NPs were prepared via two different methods; microemulsion polymerization and nanoprecipitation method. Effect of various factors such as copolymer molecular weight (M_w), PVA M_w, type of solvent and, solvent volume ratio (S/NS) and drug concentration on NP size, stability and drug loading was determined. PCLF 1250 was chosen as more suitable copolymer for its slightly higher loading of hydrophilic drug compared to PCLF 530 and also for making smaller NPs in comparison with the PCLF 2000. Drug release was also investigated in PBS. The optimum formulation was injected to the mouse for in vivo imaging to understand the possibility of delivering PCLF NPs to the lymphatic tissue. The hydrophobicity of the copolymer could help NPs accumulate into lymphatic nodes more than other tissues.