Study on oxidative pyrimidine metabolism in Rhodococcus erythropolis: Discovery and analysis of a novel midase,ureidomalonase

摘要

@@In biological system, pyrimidines are metabolized through either reductive, oxidative, or pyridine utilization (rut) pathway, and so on (Fig. 1). We applied the oxidative pathway to enzymatic synthesis of 2'-deoxyribonucleosides with purine bases from thymidine and purine bases by nucleoside phosphorylase. The introduction of oxidative pyrimidine degradation shifted the reaction equilibrium to nucleoside synthesis by removing a byproduct, thymine, and well improved the production yield. We elucidated the enzymes involved in the oxidative pathway in a bacterium Rhodococcus erythropolis JCM 3132, and clarified their physiological function.