Oxidative pyrimidine metabolism in Rhodococcus erythropolis useful for valuable nucleoside synthesis: Discovery of a novel amidohydorolase, ureidomalonase

摘要

Through the investigation of the oxidative pyrimidine metabolism in Rhodococcus erythropolis, a novel enzyme, ureidomalonase, catalyzing the final step of the metabolism was found. This enzyme catalyzed the amidohydrolysis of ureidomalonic acid, a product of barbiturase reaction, to urea and malonic acid. These finding brought a comprehensive understanding about oxidative pyrimidine metabolism for the first time, which consisted of uracil/thymine dehydrogenase, barbiturase, and ureidomalonase.