Establishment and Optimization of SRAP Conditions in C. pinnatifida Bge

摘要

The paper presents the optimal PCR conditions for sequence-related amplified polymorphism (SRAP) analysis of C. pinnatifida Bge. L16(45) orthogonal experiment with 5 factors was carried out, which resulted in 2.5mmolL-1 Mg2+, 0.15mmolL-1 dNTPs, 0.25μmolL-1 Primer, 20ng DNA, 0.5U Taq DNA polymerase being the best condition in the reaction of 20μL volume containing 1 ×PCR Buffer. Amplified products of 19 hawthorn varieties were separated using polyacrylamide gel electrophoresis. Clear and high polymorphic bands were revealed, which indicated that the optimized SRAP-PCR system can meet the need of SRAP amplification for hawthorn genome and that the SRAP markers can be used for hawthorn genetic diversity research.