Histochemical Localization of Endogenous Peroxidase in the Gill, Small Intestine, and Liver of Carassius Auratus

摘要

Peroxidases (EC 1.11.1.7) catalyze the oxidation of phenolic compounds using either hydrogen peroxide (H2O2) or organic peroxides as the oxidizing agent. Peroxidases play a significant role in the antioxidant defense systems. In immunohistochemistry, endogenous peroxidase acitivity is usually blocked with the saturating amounts of H2O2. In the present studies, the distribution of endogenous peroxidase was investigated in the gill, small intestine, and liver of Carassius auratus. The results indicate that peroxidase distributes widely in the branchial filaments and intestinal epithelial cells of Carassius auratus. However, there are fewer granules of peroxidase in the liver cells. The results indicate that it is important to block endogenous peroxidase acitivity in these tissues of Carassius auratus with immunohistochemistry technology.