Bioassay evaluation assuming multiple unknown parameters applying optimal design

摘要

Bioassays can be used to estimate the intake in accidental and routinely situations. To evaluate theeffective dose, apart from the intake, we need to know other parameters such as Activity MedianAerodynamic Diameter (AMAD) or the fraction absorption (f1) in the blood from the GI tract. In anaccident situation these parameters are often unknown. The bioassay measurement values can be used toestimate by fitting the parameters unknown. We have applied non linear regression for solving this kindof problem. Furthermore, a method to find the best moments where the bioassay measurements should betaken is described. This method is applied to optimal designs. The goodness of the design will depend onthe number of samples and the measurement accuracy. It requires obtaining the analytical solution of thebiokinetic model as a function of the parameters to be fitted. Different cases are studied using thecomputer program BIOKMOD (developed by one of the authors.