Comparative Study of LFY Homologous Genes among Citrus and Its Relative Genre Plants

摘要

LEAFY (LFY) gene not only controls flowering time and floral transition, but also plays an important role in inflorescence and floral organ development. It was situated at the central site in gene network of flowering regulation. In order to probe further the molecular mechanism among Citrus and its relative plants, complete LFY homologous genes from Satsuma (Citrus unshiu Marcovitch) , Ponkan (C. reticulata Blanco) , Shatian pomelo [ C. grandis (L.)Osbeck ] , Kumquat (Fortunella crassifolia Swingle) and Wompee [Clausena lansium (Lour.)Skeels] were isolated and cloned by polymerase chain reaction (PCR). The results showed that the nucleotide size of complete LFY homologous genes from Satsuma, Ponkan, Shatian pomelo, Kumquat and Wompee was 2 090bp, 2 086bp, 2 092bp, 2 081bp and 2 089bp respectively, and they encoded 398, 398, 398, 398 and 397 amino acids respectively. These complete LFY homologous genes were all composed of 3 exons and 2 introns. The homologies of nucleotide and amino acid sequences among the complete LFY homologous genes were 92% -99% and 95% - 100% respectively. The result of phylogenetic tree based on amino acid sequence homology among LFY homologous is similar to the result of plant taxonomy.