Determination of immunoglobulin G in human serum by means of an immunosensor fitted with an enzymatic probe as detector and a Clark electrode as transducer

摘要

A new immunosensor is developed to measure human immunoglobulin G. The method is competitive in the heterogeneous phase and separative. The greatest novelty lies in the use of a tyrosinase biosensor as detector and a Clark electrode as transducer. The enzymatic marker is alkaline phosphatase. Using this new immunosensor it was possible to determine anti-HIgG up to a concentration in the order of 10~(-11) mol l~(-1). The present note makes a detailed analytical comparison between this new immunobiosensor and a similar potentiometric detection immunosensor proposed by us in a previous work.