Production of selenium-enriched rapeseed peptides

摘要

Research has been carried out on extracting Selenium-enriched rapeseed protein isolate (RPI). Based on single factor experiment, the extraction conditions had been optimized by orthogonal analysis. The optimum conditions for phytic acid and glucosinolate extraction are that the Selenium-enriched defatted rapeseed meal is extracted 3 times with a solid to solution ratio of 1:10 at 45°C for 60 min each at a pH of 4.0. The optimum extraction conditions for Selenium-enriched rapeseed protein isolate are that the meal is extracted 3 times with a solid to solution ratio of 1:12 at 50°C for 35 min each under pH12.0. Enzyme hydrolysis for Selenium-enriched rapeseed protein isolate has been carried out. An alkaline enzyme, alcalase, was selected to hydrolyze the Selenium-enriched rapeseed protein isolate. The substrate (having a concentration of 3%) was digested with alcalase (180000U/g) for 7 hours at 50°C and a pH of 8.0. Under this condition, 32.51% of the substrate is hydrolyzed. The nitrogen recovery rate is 91.28%. The crude production is then decolorized with active carbon and desalinized, concentrated and lyop hilized. Molecular weight of Selenium-enriched rapeseed peptide is lower 1500 Dalton.