Studies on transferring △6- fatty acid desaturase gene(D6D) into Brassica napus L. plants

摘要

The desaturase of △6-fatty acid (D6D) is a rate-limiting enzyme in the production of γ-linolenic acid(GLA) and it can converts linoleic acid(LA) into α-linolenic acid(ALA) and GLA. In this study, a △6-fatty desaturase gene was cloned from Rhizopus stolonifer and the plasmid pCNR was constructed by inserting D6D gene into plant high-efficient expression vector pCAMBIA2301G. This construct was used for Brassica napus L. transformation mediated by Agroubacterium tumefaciens LBA4404. Results from GUS assay and PCR analysis oftransformed plants indicated that △6-fatty acid desaturase gene from the Rhizopus nigricans was integrated into Brassica napus genomes.