Molecular markers for the seed coat color in Brassica juncea§(B3)

摘要

The yellow-seeded rapeseed has many advantages over the brown-seeded one, such as higher oil content, higher feeding value, and better market value. To elucidate inheritance of seed coat color in Brassica juncea. the Sichuan Yellow inbred (PY)was crossed with the Ziyejie inbred and their F1, F2 and BC1 and BC2 progenies derived from backcrossing to PY were phenotyped for seed coat color. Results showed that the yellow seed coat was controlled by two independent recessive loci. The seven brown-seeded near-isogenic lines were developed by successive backcrosses to PY and selfing. One of the BC5F2 populations segregating for a single locus controlling seed coat color was used for mapping. Using the 88 primer pairs of sequence-related amplified polymorphism and the 500 random primers, two markers were found to be linked to the gene for brown seed coat, which designated as SCM57 and SCM1078 The crossover between these markers and the brown seed coat locus was 235% and 7.06%, respectively. The markers were located at the same side of the brown seed coat locus, and 2.41 and 7.51cM away from the locus. They are located at the same linkage group of the marker RA2-A11 previously published by Padmaja et al. (2005).