Preliminary study on mutagenic effects of 60Co-γand spaceflight treatment to Brassica napus

摘要

40 double-low seeds of Brassica napus were irradiated by γ-ray at dose of 800Gy, 1000Gy and 1200Gy respectively. 12 lines were experienced the spaceflight by "Shenzhou 4" and "Round-trip artificial satellite No. 18". Molecular markers detection were performed and the agronomic characters were investigated in M1 (radiation contemporary), SP2, SP3 (progeny of plant carried by spaceflight). The main results are as follow ( 1 ) the seedling survival rate, Plant height, height of primary branch, No. of silique per plant and seed weight per plant in M1 significantly decreased comparing with the control, but the difference on 1000-seed weight was not significant. There are Polymorphism of characteristic band on SSR primer Na-OE02 between M1 and the control. Early-flowering plant and dwarf plant appeared in M1. (2) SP1 had no obvious effect in germination percentage, seedling survival rate, seed setting and other agronomic characters. Some mutated plants appeared in SP2 however. Useful mutants included the plants with long silique, more pods, dwarf plant, later maturity and bigger seeds et al. And the results of identifying agronomic characters of the mutants in SP2, SP3 indicated that the mutated characters of more branches, more pods, and bigger seeds could be heritable from SP2 to SP3. The later maturity and more pods mutated plant had characteristic band comparing with the control on SSR and RAPD primer.The same to its SP3 plants. It indicated that the spaceflight mutagenesis produced hereditable mutation.