Changes of Tau protein's expression during the differentiation process of bone-marrow MSCs into neurons

摘要

The aim of this study was to explore the expression changes of Tau, pSer202, and Tau's contents during cavy bone-marrow mesenchymal stem cells (MSCs) differentiation into neuron by EGF and bFGF in vitro. Phenotypic changes were monitored by the inverse phase-contrast microscopy. Expression changes of Tau and pSer202 were tested by immunocytochemistry, and Tau's content was determined by ELISA. Positive cells rates and contents of Tau were low before differentiation; but after induction, the cellular shapes of MSCs became very similar to neurons; positive rates of Tau and NSE increased significantly (P < 0.05) compared with the uninduced groups. ELISA indicated that Tau's contents in all passages were low in early phase of differentiation and enhanced gradually during the differentiation process, which became steady after 10-day induction. P4-, P8-, P12-, P16-MSCs had the same change tendencies by cytokines. EGF+bFGF had a greater effect on the expression changes of Tau. pSer202 hadn't expressed in uninduced and induced groups. It is concluded that there was an upward tendency in Tau expression and non-phosphorylated state at pSer202 during MSCs differentiation into neural cells by EGF and bFGF.