Preparation and characterization of the peptide modified regenerated cellulose membrane for binding H1N1 virus

摘要

The peptide modified regenerated cellulose membrane was prepared by epoxy method. To characterize the membrane, Atomic Force Microscope (AFM) was used to observe the membrane structure, and measure the adhesion force between the nano-gold coated tip and the membrane. For epoxy group grafting, the regenerated cellulose membrane was modified at various pH, epichlorohydrin(EPI) concentration, dimethyl sulfoxide (DMSO) concentration, and reaction temperature. High pH, EPI concentration, and temperature can enhance the grafting process. However, when higher than critical values of reaction temperature and pH are used, the regenerated cellulose membrane is hydrolyzed. For peptide grafting, the grafting content was from 0.02 mg/cm~2 to 0.06 mg/cm~2 of Met-Enkephalin peptide at various pH buffers. In spite of large amount of peptide grafted, the peptide activity is destroyed at high reaction pH, which resulted in the decreased A/WSN/33 virus attachment. The AFM adhesion force curve was used to explain the behavior of the peptide modified regenerated cellulose membrane. The peptide modified regenerated cellulose membrane was also analyzed by FTIR.