Repeated Solid-Phase Fermentation and Extraction for Enzyme Production

摘要

Solid-phase fermentation has been found to have a much higher productivity than the popular liquid submerged fermentation in producing cellu-lase enzymes. The highest reported productivity in the literature for cellulases by Trichoderma cultures in submerged fermentation is 158 filter paper units (FPU) / (h*L) of fermenting liquid. From preliminary experiments of solid-phase fermentation in 1000-mL flasks, a productivity of 234 FPU of cellulases/(h*L) of solid-bed volume was obtained. When two novel techniques―pressure pulsation and repeated extraction―were applied, a productivity of 806 FPU/(h*L) was achieved. The same techniques also greatly enhanced the productivity of other enzymes by fungal cultures in solid-phase fermentation.