Real-time analysis of xenobiotic degradation and microbial interactions in a bioaugmentation system by bioluminescence detection technique

摘要

A newly created bioluminescent Pseudomonas putida BLU harboring TOL plasmid was used to quantitatively examine its growth and bioluminescence output by degrading m-toluic acid (m-TA) as the sole carbon source in a mixed culture with the wild strain of P. putida mt-2 and in activated sludge. In the activated sludge introduced with the bioluminescent strain, the bioluminescence output corresponded well with the degradation of m-TA for a long-period fill and draw culture, showing the bioluminescent m-TA-degrader can be well incorporated into the activated sludge. In the mixed culture of P. putida BLU with the wild strain at different concentration ratios but the total inoculum size remaining the same, the bioluminescence (LUX) was quantitatively dependent upon the inoculated P. putida BLU concentration, while correlated to the total cell concentration (X) by LUXpropor.to X~(1.6) in the growth phase for all the cases. However, in the growth phases in the activated sludge, the bioluminescence was correlated with the total cell concentration by LUX propor.toX~(3.8-7.0), varying with culture time. This result suggested tha the microbes of the activated sludge have interacted with the P.putida BLU.