Establishment of ln-vitro ~(60)Co Dose Calibration Curve for Dicentrics in National Biodosimetry Laboratory of Malaysia

摘要

The purpose of this study was to establish an in-vitro ~(60)Co dose calibration curve for dicentric assay technique. Sample from six volunteers were irradiated using ~(6S)Co at doses of 0, 0.25, 0.5, 1.0, 2.0 and 4 Gy. The blood samples were surrounded by 4 mm Polyvinyl chloride (PVC). These were irradiated at a dose rate of 0.98 Gy min~1. Blood specimens were then cultured and processed using dicentric assay technique. The observed dose calibration data were fitted to a linear quadratic model using Dose Estimate Ver 2.0, established by Health Protection Agency, UK. The calibration curve parameters were compared with results from other studies. The dose calibration curve for dicentrics yield is: Yield = (0.0010 ± 0.0000) + (0.0899 ± 0.0002)D + (0.0185±0.0001 )D2, (Weighted X~2 = 7.4510, on 3 df, P=0.05). The value of a was higher, while P value was lower than the other reported studies. We have established a dose calibration curve for the induction of chromosome aberrations in human lymphocytes from blood irradiated with ~(6S)Co —ray in a dose range of 0.0-4.0 Gy. This curve may be useful for in- vitro dose reconstruction.