Comparison of dicentric dose response curves of 6 MV LINAC X-rays and Co-60 gamma-rays for biodosimetry application

摘要

In vitro Dicentric (DC) dose response curves of 6 MV X-rays (3 Gy/min) and Co-60 gamma-rays (0.63 Gy/min) were generated and compared (intra-laboratory) to understand their similarities and differences. Human peripheral blood samples exposed to ten different doses (0, 0.05, 0.1, 0.25, 0.5, 1, 2, 3, 4 and 5 Gy) of 6 MV X-rays and 60Co gamma-rays were subjected to Dicentric Chromosome Assay (DCA) and dicentrics, excess acentric fragments (AF) and chromatid breaks (CB) were scored. Totally about 31,553 metaphase spreads were scored for the purpose. Dose response curves of both radiation qualities were almost same except for a 13.8% higher 13 value for 6 MV X-rays. However, blind tests results revealed that both these curves are biologically equivalent and exhibited good dose prediction accuracy for the entire dose range. This demonstrated the feasibility of interchangeable use of these curves in biodosimetry. Consequently it has been suggested that LINAC facilities worldwide can be roped in for biodosimetry capacity augmentation towards managing nuclear emergency situations involving gamma-radiation exposures.