Biosynthesis and properties of an extracellular metalloprotease from the Antarctic marine bacterium Sphingomonas paucimobilis

摘要

An extracellular protease from the marine bacterium Sphingomonas paucimobilis, strain 116, isolated from the stomach of Antarctic krill, Euphausia superba Dana, was purified and characterized. The exretion of protease was maximal at temperatures from 5 to 10 deg C, i.e. below the temperature optimum for the strain growth (15 deg C). The highly purified enzxyme was a metalloprotease (sensivity to ethylenediaminetetraacetic acid (EDTA)) and showed maximal activity against proteins at 20-30 deg C and pH 6.5-7.0, and towards N-benzoyl-tyrosine ethyl ester (BzTyrOEt) at pH 8.0. At 0 deg C the enzyme retained as much as 47