Cell cultures form marine invertebrates: obstacles; new approaches and recent improvements

摘要

The establishment of cell lines from marine invertebrates has been encountered with obstacles. Contrary to insects and arachnids where the development of a variety of cell lines has become routine, thee is no single established cell line from marine invertebrates. This review examines the activity in the field of marine invertebrate cell cultures within the last decade (1988-1998). During this period, Attempts (90 peer reviewed studies in addition to many other abstracts, chapters in books, symposia presentations and reports) were limited to a few species within only six phyla (porifera, chapters in books, symposia presentations and reports) were limited to a few species within only six phyla (Porifera, Cnidaria, Crustacea; Mollusca, Echinodermata, Urochordata; in addition to freshwater/terrestrial annelids and platyhelminths) . These studies which are summarized here, on one hand indicated ubiquitous problems and on the other, unique charcterizatons to each phylum studied. Only one-third of the studies revealed cultures of 1 month or longer but most of these were long-term cultures found or suspiciouly considered to be contaminated by othe runicellular eukaryotic orgnaisms, mainly by thraustochytrids. Three unique approaches/obstacles for marine inverte-bratecell cultures (sourece of cell, cryopreservation and eukaryotic contaminants) are further discussed. The overall impact of recent improvements and developed protocols raises the suggestion for testing different, novel routes in the establishment of cell cultures from marine invertebrates. direct 1999 Elsevier Science B.V. All rights reserved.