Polymorphism of the IL1B, NOS3, GSTT1 and GSTM1 Genes and ChromosomalAberration Levels in Peripheral Blood Lymphocytes of the Siberian Group ofChemical Enterprises Workers

摘要

Individual radiosensitivity (IR) in man is a complex trait which is developed under genetic and environmentalcontrol. Analysis of inherited basis of IR is an urgent issue which solution will be beneficial for the understanding offundamental mechanisms of intrinsic and extrinsic factors interaction in the development of complex phenotypes and fordevelopment of strategy of genotype-specific diagnosis of individual response to ionizing irradiation at radiation-dangerousenterprises.In 141 workers of chemical-metallurgical plant of the Siberian Group of Chemical Enterprises with established dose ofexternal γ-irradiation, association between the polymorphisms of the interleukin-1beta (IL1B, +3953A1/A2), endothelialNO-synthase (NOS3 VNTR in 4th introne), glutathione S-transferases teta1 and mu1 (GSTT1, GSTM1, null and normal alleles)genes and levels of chromosomal aberrations (CA) as markers of IR was investigated.With use of standard cytogenetic analysis it was established that mean levels of all cytogenetic signs, including radiationexposition markers (dicentrics and rings), in the group studied met spontaneous levels. It was shown that homo- andheterozygous carriers of NOS3*VNTR4A allele have higher levels of chromatid type CA as compared to the homozygotes"B/B" (0,86%, 1,09% and 1,19%, respectively; p<0,05). Also, "A/A" genotype of the NOS3 gene was associated with elevatedrates of dicentrics and rings. It is likely, that the NOS3*VNTR4A allele, which links to increased levels of NO in blood, isassociated with more marked effects of radiation exposition and higher levels of IR.The data obtained confirm that genetics factors significantly influence the IR in man, and NOS3 gene has outstanding role inthis respect.