Protein Denaturation by Ionic Liquids and the Hofmeister Series

摘要

We have used differential scanning calorimetry (DSC) for studying how ionic liquids affect the thermal stability of proteins.Most 1,3-dialkylimidazolium, 1,1-dialkylrnpyrrolidinium and tetraalkylammonium salts decrease the thermal stability, but some protic ionic liquids stabilise the native state. The effects can be systematised by the Hofmeister ion series. Depending on the nature of the ions, ionic liquids can also suppress the formation of misfolded proteins and the irreversible deactivation of proteins at high temperatures.