A highly sensitive chemiluminescence method for earlydiagnosis of radiation damage

摘要

Chemiluminescence (CL) is a highly sensitive detection method with broad biological applications. In this work, a CL detection system is developed and applied in detection the CL from human lymphocyte induced with Co-60 irradiation, for the first time. The radiation damage of lymphocyte was detected by cell counting and MTT method for various irradiation doses. The results show a good correlation between the CL intensity and the radiation-induced damage of lymphocyte. Cell activity increased gradually with the increase of radiate dose, when the dose was under 3Gy. When the radiate dose was above 3Gy, the results were contrary. The cell counting results corresponded well with the MTT method. The CL detection method, thus, may provide an alternative way in evaluation of radiation damage.