Advanced multi-focus confocal laser scanning microscope for single molecule studies

摘要

A new multi-focus multi-confocal set-up for performing fluorescence spectroscopy of single molecules in solution is presented. The ultimate goal of the set-up is to track individual molecules during diffusion in solution, when all standard methods of trapping such as optical tweezers or dielectrophoretic traps fail. We present here a detailed description of the experimental setup and show first experimental results.