THE CRYOPRESERVATION OF MICE SKINS FOR 5 WEEKS AT −20 ℃ AND OF HUMAN SKIN FOR 33 YEARS AT −196 ℃

摘要

Experimental and clinical studies of cryopreservation of mouse skin at −20 ℃and of human skin at −196 ℃. Tail skin of C3H / He mouse was excised and wrapped in the sterile gauze (3 g in weight) and it was kept in the small dish (100 ml in capacity), which was moisten of 100 ml of physiologic saline added 50 mg of Panimysin® and did not include any cryoprotectant. The temperature of the ice box was continuously recorded by the thermistor. During freeze-preservation for five weeks, the highest and the lowest temperature were −10 and −28 ℃respectively, and the average was −20 ℃. The stored skin was transplanted to the skin defect (1 cm~2) created on the back of another C3H / He mouse by the non-suture technique. The macroscopic and microscopic examinations were done from second seek to 8th week after the surgery. Human allogeneic skin graft was harvested from the amputated leg, and cryopreserved in 8 % glycerol solution in TC 199 for 14 days at −196 ℃. The graft was transplanted onto the ulcer surface from the left mastectomy.rnThe transplanted mouse skin was taken for 6 months. Human skin grafts stored for 2 weeks at −196 ℃under the optimal conditions were taken too, and each methods will be clinically available. The human adipose tissues frozen for 33 years showed confluent MSCs by RFTIVIVSCM, which will be used for long-term cryopreservation of skin, subcutaneous and perivenous fat tissue.