ATPィメージセンサのィメー ジングに及ぼす酵素パターンから 拡散するATP及びプロトン分子の影響解析

摘要

To detect a neurotransmitter in the nervous system simultaneously, we developed a pattering technique using a cross-linkable polymer to immobilize apyrase on a charge coupled device (CCD)-type image sensor. An apyrase-immobilized membrane was patterned by the proposed technique and characteristics of the fabricated ATP-image sensor are reported in our previous report. The bio-image sensor determines ATP concentrations by measuring the formed H+ ions from an enzymatic reaction in real-time. However, on the experiment, it was difficult to distinguish between the produced H+ions and the other H+ ions that are diffused from nearby membranes or other origins. In this study, the diffusion characteristics of H+ions for bio-image sensor was measured and evaluated. The results used to determine a measurement time that can minimize the undesirable impact of the diffused ions.