A Quantitative Label-Free Displaying Method of Neuronal Firing via Optical Phase Information

摘要

, on both sides of the cell membrane. That is, the ion concentration in the cell body has large change during the generation of action potential. The variation of ion concentration will further affect the refractive index and the phase distribution of a sample is affected by the related refractive index distribution. Therefore, the variation of phase is actually induced by the variation of ion concentration in the cell body. In other words, the neuron firing can be described via optical phase information. To explore the correlation between the neuronal firing activities and the variation of optical phase, a phase imaging experiment is designed and implemented. Firstly, the phase distribution of the simulation model in the solution with different concentration is obtained. Then, the phase value of sampling point in the solution of different concentration is obtained and the phase has linear relation with Na