Detection of Relevant Mycotoxins in Wheat Beer and Sake by LC-MS/MS Using Prototype Immunoaffinity Column Clean-Up: A Preliminary Study

摘要

All of 12 relevant mycotoxins, namely deoxynivalenol (DON), aflatoxins B_1, B_2, G_1 and G_2, ochratoxin A (OTA), fumonisins B_1, B_2 and B_3, T-2 toxin (T-2), HT-2 toxin (HT-2), and zearalenone (ZON) present in complex wheat beer and sake matrices were extracted using multifunctional mixed bed immunoaffinity columns. They were detected using a LC-MS/MS method that achieved good separation and detection. Recoveries of all the toxins from the immunoaffinity columns ranged from 64-127%. The LC separation was performed using a reverse phase C18 column. MS measurements were done in both the positive and negative ESI modes. Multiple reaction monitoring (MRM) was carried out for each compound.