Probing Fluoresce Upon Hybridization on Molecular Beacons with Conjugated Inorganic Quantum Dots

摘要

Since the first report on molecular beacons(MBs) have been developed by Tyagi and Kramer in 1996, MBs have became a class of DNA probes that is widely used in chemisty, biology, biotechnology and medical sciences for biomolecular recognition. Here, we introduced semiconductor quantum dots(QDs)-conjugated MBs that composed of a 24-base-long single-stranded DNA (ssDNA) molecule, a CdTe QDs and a fluorophore that could highly quench the NPs through the fluorescence resonance energy transfer(FRET). The loop section of MBs was formed by 16-base DNA. CdTe QDs was connected through the amide bond to the 5' end of the MBs and a quenching molecular, such as 4-((4'-(dimethylamino) phenylazo ) benzoic acid (DABCYL) connected to the 3' end of the MBs(Fig.1).