Double wavelength multifunctional optical tweezers

摘要

We report the comprehensive application of double wavelength multifunctional optical tweezers. In the presented setup the measurement system is based on an inverted biological microscope. The system is equipped with two lasers of different wavelengths. The lasers' beams, which generated optical traps, are controlled by galvanometer mirrors systems. Application of this setup has been presented in three examples. First one describes measurement of liposomes' deformation, which is widely utilized in medical research. Secondly, the application of double wavelength multifunctional optical tweezers in precise control of micro-tools such as micro-dumbbells is presented. The third application of this system is the measurement of cell-to-cell adhesion forces, what is essential to understand the physical characteristics of living cells. In this paper we described preparation and process of samples measurement.