Nano-scale optical imaging technique

摘要

The optical resolution limits the observation of fine features with size below diffraction limit, e.g. 500nm. We propose and demonstrate a technique that uses collimated laser scattering to characterize such features. We use a new illumination technique that let us image bio-molecules down to 50 nm with optical microscopy. We observed vesicles with size down to 50 nm and visualize the flow of such tiny molecules in the live cells. We could also characterize the detailed size and molecular weight of such particles.