Application of fluorescence spectroscopy for on-line bioprocess monitoring and control

摘要

Modern bioprocess control requires fast data acquisition and in-time evalaution of bioprocess variables. On-line fluorescence spectroscopy for data acquisition and the use of chemometric methods accomplish these requirements. The presented ivnestigations were performed with fluorescence spectrometers with wide ranges of excitation and enission wavelength. By detection of several biogenic fluorophors (amino acids, coenzyms and vitamins) a large amount of information about the state of the bioprocess are obtained. For the evalaution of the process variables partial least squares regression (PLS) is used. this technique was appleid to several bioproceses: the production of regotamine by Claviceps purpurea, the production of t-PA (tissue plasminogen activator) by animal cells and brewing processes. The main point of monitoring the brewing processes was to determine the process variables cell count and extract concentration.