METHOD AND DEVICE ASSEMBLY FOR PREDICTING A PARAMETER IN A BIOPROCESS BASED ON RAMAN SPECTROSCOPY AND METHOD AND DEVICE ASSEMBLY FOR CONTROLLING A BIOPROCESS

摘要

A method of predicting a parameter of a medium to be observed in a bioprocess based on Raman spectroscopy comprises the steps of: acquiring a first series of preparatory Raman spectra of an aqueous medium using a first measuring assembly; normalizing the first series of preparatory Raman spectra based on a characteristic band of water from at least one Raman spectrum acquired with the first measuring assembly; building a multivariate model for the parameter based on the normalized preparatory Raman spectra; acquiring predictive Raman spectra of the medium to be observed during the bioprocess with another measuring assembly; normalizing the predictive Raman spectra based on a characteristic band of water from at least one Raman spectrum acquired with the other measuring assembly; and applying the built model to the predictive Raman spectra for predicting the parameter. A device assembly for predicting a parameter of a medium to be observed in a bioprocess is adapted to carry out this method.