Effects of Five Kinds of Antibiotics on the Growth of Skeletonema costatum

摘要

实验室条件下,研究了青霉素(Amipicillin)、硫酸庆大霉素(Gentamycin sulfate)、硫酸新霉素(Nomycin sulfate)、氯霉素(Chcoramphencol)、硫酸卡那霉素(Kanamycin sulfate)对中肋骨条藻(Skeletonema costatum)生长的影响.结果表明:较低浓度的青霉素(0.05mg/mL)、硫酸新霉素(0.05mg/mL)和硫酸卡那霉素(0.05-0.50mg/mL)对中肋骨条藻的生长有明显的促进作用；而较高浓度的青霉素(0.50-2.00mg/mL)、硫酸庆大霉素(0.10-2.00mg/mL)、硫酸新霉素(1.00-2.00mg/mL)、氯霉素(0.05-2.00mg/mL)、硫酸卡那霉素(2.00mg/mL)对中肋骨条藻的生长有明显的抑制作用,随着浓度的增加抑制作用愈加明显.2.00mg/mL的青霉素、硫酸庆大霉素、硫酸新霉素、氯霉素、硫酸卡那霉素处理10d的中肋骨条藻的密度分别是对照组的55.25％、59.70％、53.39％、15.71％、91.95％,中肋骨条藻对5种抗生素的敏感性存在明显的差异.实验结果为评估抗生素对海洋生态系统的影响提供依据.%The effects of Amipicillin,Gentamycin sulfate,Nomycin sulfate,Chcoramphencol,Kanamycin sulfate on the growth of Skeletonema costatum were studied under laboratory conditions.The results showed that Amipicillin(0.05mg/mL),Nomycin sulfate(0.05mg/mL) and Kanamycin sulfate(0.05-0.50mg/mL) could stimulated the growth of Skeletonema costatum under lower concentrations,and the growth stimulation of Skeletonema costatum was significant (p＜0.05).Amipicillin(0.50-2.00mg/mL),Gentamycin sulfate(0.10-2.00mg/mL),Nomycin sulfate(1.00-2.00mg/mL),Chcoramphencol(0.05-2.00mg/mL) and Kanamycin sulfate(2.00mg/mL) could inhibited the growth ofSkeletonema costatum under higher concentrations.The growth inhibition was significant (p＜0.05) and more obvious with increased concentration of antibiotics.Compared with the control group,the percentage of cells density of Skeletonema costatum which was treated by Amipicillin,Gentamycin sulfate,Nomycin sulfate,Chcoramphencol,Kanamycin sulfate was 55.25％,59.70％,53.39％,15.71％,91.95％ respectively under 2.00mg/mL on the 10th day.Skeletonema costatum showed the significant difference in sensitivity to five kinds of antibiotics.The experimental results would provide assessment foundation for the effects of antibiotics on marine ecosystems.