小窝蛋白-1对血管内皮生长因子孵育的骨肉瘤细胞血管内皮生长因子受体-2表达水平的影响

摘要

目的:研究小窝蛋白-1(caveolin-1)对血管内皮生长因子(VEGF)孵育的骨肉瘤细胞株(SOSP-9607)血管内皮生长因子受体-2(VEGFR-2)表达的影响,探讨其在骨肉瘤发展中作用的可能机制.方法:实验分为三组,即对照组、VEGF组、VEGF+caveolin-1 小片段干扰核糖核酸(siRNA)组.反转录聚合酶链式反应(RT-PCR)检测VEGF孵育的SOSP-9607细胞中caveolin-1、VEGFR-2信使核糖核酸 (mRNA)的表达;蛋白质印迹(Western Blot)检测caveolin-1、VEGFR-2蛋白的表达.结果:与对照组相比,VEGF组细胞caveolin-1、VEGFR-2 mRNA和蛋白表达明显增加(均P<0.01),VEGF+caveolin-1 siRNA组细胞caveolin-1 mRNA和蛋白表达减少,而VEGFR-2 mRNA表达增加,蛋白水平却表达减少,差异具有统计学意义(P<0.05).结论:caveolin-1能明显减少VEGF刺激下的骨肉瘤细胞中的VEGFR-2蛋白水平的表达,提示caveolin-1在VEGF依赖性信号级联反应中起正调控作用,为临床选择合适的靶点治疗骨肉瘤的血管生成提供实验依据.%Objective: To observe the effect of caveolin-1 on the expression of VEGFR-2 in VEGF-incubated osteosarco-ma cells and to explore the possible mechanism of its role on osteosarcoma development. Methods: There were three groups, control group, VEGF group, VEGF+caveolin-1 siRNA group. The expression of caveolin-1, VEGFR-2 mRNA and protein were detected with RT-PCR and Western Blot. Results: Compared with the control group, the expression of caveolin-1, VEGFR-2 significantly increased in VEGF group (all P<0.01). Compared with the control group, the expressions of mRNA and protein of caveolin-1 decreased, while the expression of mRNA of VEGFR-2 increased, and protein was reduced in VEGF+caveolin-1 siRNA group, there was a significant difference (P<0.05). Conclusion: caveolin-1 can significantly reduce the expression of VEGFR-2 in protein level in VEGF-stimulated osteosarcoma cells, suggesting that cave -olin-1 plays a positive regulatory role in VEGF-dependent signaling cascades. It can be the appropriate target for clinical treatment angiogenesis in osteosarcoma and provide an experimental basis.