The new image fusion method applied in two wavelengths detection of biochip spot

摘要

In the biological systems genetic information is read, stored, modified, transcribed and translated using the rule of molecular recognition. Every nucleic acid strand carries the capacity to recognize complementary sequences through base paring. Molecular biologists commonly use the DNA probes with known sequence to identify the unknown sequence through hybridization. There are many different detection methods for the hybridization results on a genechip. Fluorescent detection is a conventional method. The data analysis based on the fluorescent images and database establishment is necessary for treatment of such a large-amount obtained from a genechip. The unknown sequence has labeled with fluorescent material. Since the excitation and emission band is not a theoretical narrow band. There is a different in emission windows for different microscope. Therefore the data reading is different for different microscope. We combine two narrow band emission data and take it as two wavelengths from one fluorescence. Which by corresponding UV light excitation after we read the fluorescent intensity distribution of two microscope wavelengths for same hybridization DNA sequence spot, we will use image fusion technology to get best results * We introduce a contrast and aberration correction image fusion method by using discrete wavelet transform to two wavelengths identification microarray biochip. This method includes two parts. First, the multiresolution analysis of the two input images are obtained by the discrete wavelet transform, from the ratio of high frequencies to the low frequency on the corresponding spatial resolution level, the directive contrast can be estimated by selecting the suitable subband signals of each input image. The fused image is reconstructed using the inverse wavelet transform. The second method is to correct the aberration; here we correct distortion aberrations with distortion aberration equation, which shows the deviation is proportional to the cube of image high. By compare the object of the standard square matrix and the distortion image we can reduce the distortion aberration. This technique is relevant to visual sensitivity and aberration of two wavelengths fluorescence microscope to detect the biochip microarray. The results show that the fused image can get better analysis of the details of each original micro spot biochip.