Quantitative estimation of absorbing chromophores in tissue simulators based on laser induced spectroscopy and scattering measurements

摘要

The determination of the tissue optical parameters and the light propagation in multicomponent tissues is an important tool for real-time laser treatment dosimetry (selective laser ablation, PDT) and non-invasive laser diagnostics (laser induced fluorescence spectroscopy, optical tomography). The aim of this work is the experimental study of the optical characteristics of laser light propagation in tissue simulators, employing spectroscopic techniques, in order to quantify the concentration of tissue chromophores. Total and diffuse reflectance/transmittance measurements of tissue phantoms were performed at visible wavelengths and the calculated optical properties were correlated to the concentration of the absorbing species in a multicomponent environment. The experimental results demonstrated the ability of the laser induced spectroscopic techniques for quantitative estimation of tissue chromophores concentration.