Using broadband spatially resolved NIRS to assess muscle oxygenation during altered running protocols

摘要

We used spatially resolved near-infrared spectroscopy (SRS-NIRS) to assess calf and thigh muscle oxygenation during running on a motor-driven treadmill. Two protocols were used: An incremental speed protocol (velocity = 6-12 km/h, Av = 2 km/h) was performed in 3 minute stages, while a pacing paradigm modulated step frequency alternatively (2.3 Hz [S_(Low)]; 3.3 Hz [S_(High)]) during a constant velocity for 2 minutes each. A SRS-NIRS broadband system (600 - 1000 nm) was used to measure total haemoglobin concentration and oxygen saturation (SO_2). An accelerometer was placed on the hip joints to measure limb acceleration through the experiment. The data showed that the calf (SO_2 58 to 42%) desaturated to a significantly lower level than the thigh (61 to 54%). During the pacing protocol, SO_2 was significantly different between the S_(low) vs- S_(high) trials. Additionally, physiological data as measured by spirometry were different between the S_(low) vs. S_(High) pacing trials (VO2 (2563± 586 vs. 2503 ± 605 Ml/min). Significant differences in VO2 at the same workload (speed) indicate alterations in mechanical efficiency. These data suggest that SRS broadband NIRS can be used to discern small changes in muscle oxygenation, making this device useful for metabolic exercise studies in addition to spirometry and movement monitoring by accelerometers.