Optoelectronic method for identifying Fentanyl and the main metabolite Norfentanyl

摘要

This article highlights using liquid chromatography and the MRM method, the presence of fentanyl and norfentanyl [2] in a biological sample, in this case urine. In the method you can see the presence of each segment in the ion of the substance, obtained by ElectroSpray ionization, for which the confirmation is desired. The time of acquisition and the potential collision used to obtain it are specified. The MRM method is performed with a varying concentration gradient of between 10% and 65% over a 9 minute acquisition time at a constant flow rate of 0.3 ml / minute. The gradient increases from 10% to 40% in the first two minutes, then increases from 40% to 65% over the next 3 minutes. The 65% concentration remains for the next 4 minutes.