In vivo Raman spectroscopy integrated with multimodal endoscopic imaging for early diagnosis of gastric dysplasia

摘要

A near-infrared (NIR) Raman spectroscopy system integrated with multimodal endoscopic imaging has been developed for the early noninvasive in vivo diagnosis and detection of gastric malignancies. High-quality in vivo Raman spectra in the range 800-1800 cm~(-1) can be acquired from gastric normal and premalignant (dysplastic) mucosal tissue within 1 second under the guidance of white-light and narrow-band gastroscopic imaging during clinical gastroscopy. Prominent differences in Raman spectral shapes and intensities are observed between normal and dysplastic gastric mucosal tissue, particularly in the spectral ranges 800-900, 1250-1450 and 1600-1800 cm~(-1), which primarily contain signals related to proteins, nucleic acids and lipids. The empirical intensity ratio algorithm I_(875)/I_(1450) identifies dysplasia from normal gastric tissue with a sensitivity of 100% and specificity of 100%. Our initial investigations show that in vivo NIR Raman spectroscopy in conjunction with multimodal endoscopic imaging modalities holds a great promise for improving the early diagnosis and detection of gastric premalignancies.