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POLYMER GRIPPERS AS END-EFFECTORS FOR BIOLOGICAL SAMPLE MANIPULATION

机译:聚合物抓取器作为生物样品处理的最终效应物

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摘要

We report the development of completely releasable SU-8 based polymer microgripper and the manipulation of normal rat kidney (NRK) cells suspended in phosphate buffered saline (PBS) solution using a generic biological sample manipulator, which incorporates such a polymer microgripper as an end-effector. The electrically insulative polymer gripper consists of a thick (~50 μm), patterned high aspect ratio (~5:1) layer of SU-8 as the structural layer and a thin nickel layer as the electrothermal heating layer. The fabricated polymer gripper was completely released from the substrate and mounted onto a ceramic pad. The gripper was characterized in air and PBS, and the displacement at the tips was 12 μm for 0.5 V in air and for 2 V in PBS. The mounted gripper was assembled as end-effector onto a biological nano-manipulator (L200, Zyvex Corporation, Richardson, TX). Pick-and-place of a single cell from a cluster of suspended cells in aqueous medium has been demonstrated using this set-up.
机译:我们报告了使用通用生物样品操纵器开发的完全可释放的基于SU-8的聚合物微夹钳以及悬浮于磷酸盐缓冲盐水(PBS)溶液中的正常大鼠肾脏(NRK)细胞的操作,该操纵器结合了这种聚合物微夹钳作为末端-效应子。电绝缘聚合物夹持器由厚的(〜50μm),带图案的高长宽比(〜5:1)的SU-8层作为结构层和薄的镍层作为电热加热层组成。所制成的聚合物夹持器从基材上完全释放出来,并安装在陶瓷垫上。夹具的特征是在空气和PBS中,在空气中0.5 V和在PBS中2 V时,尖端的位移为12μm。将安装好的夹具作为末端执行器组装到生物纳米操纵器(L200,Zyvex Corporation,Richardson,TX)上。使用这种设置已经证明了从水性介质中的悬浮细胞簇中提取单个细胞。

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