首页> 外文会议>Asia Pacific Conference on Optics Manufacture; 20070111-13; Hongkong(CN) >Novel Design of Confocal Fluorescence Detecting System for Capillary array and Microchip DNA fragment analysis
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Novel Design of Confocal Fluorescence Detecting System for Capillary array and Microchip DNA fragment analysis

机译:用于毛细管阵列和芯片DNA片段分析的共聚焦荧光检测系统的新设计

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摘要

A novel multi-channel DNA fragment analysis system is presented, which is based on single PMT confocal fluorescence detection and optical scanning adopting an f-theta lens. Capillary electrophoresis experiments were executed in the system for DNA marker pBR322/Hae III. Signal was processed with wavelet denoising. The system's limit of detection (dsDNA with the probe of Thiazole Orange, TO) was evaluated to be 1.1841 × 10~(-11)mol/L. Its working noise is much lower compared with that based on mechanical scanning one, and the stability and detecting sensitivity is high. The system was expected to be applied to both capillary array and microchip electrophoresis detection based on laser-induced fluorescence.
机译:提出了一种新颖的多通道DNA片段分析系统,该系统基于单PMT共聚焦荧光检测和采用f-theta透镜的光学扫描。在系统中对DNA标记pBR322 / Hae III进行了毛细管电泳实验。用小波去噪处理信号。该系统的检出限(用噻唑橙(Thiazole Orange),TO)测得的dsDNA评估为1.1841×10〜(-11)mol / L。与基于机械扫描的噪声相比,它的工作噪声要低得多,并且稳定性和检测灵敏度都很高。该系统有望应用于基于激光诱导的荧光的毛细管阵列和微芯片电泳检测。

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