Effect of electromagnetic pulse exposure on the permeability of blood-testicle barrier in mice

摘要

AIM To study the effect of electromagnetic pulse (EMP) exposure on the permeability of Blood-testicle barrier(BTB) in mice. METHODS Adult male BALB/c mice were exposed to EMP at 200kV/m for 200 pulses with 2 seconds interval. The mice were injected with 2% Evans Blue solution through caudal vein at different time point after exposure, and the permeability of BTB was observed by fluorescence microscope. The testis sample for the transmission electron microscopy was prepared at 2h after EMP exposure. The permeability of BTB in mice was observed by using Evans Blue tracer and lanthanum nitrate tracer. RESULTS After exposure, cloudy Evans Blue was found in the testicle convoluted seminiferous tubule of mice. Lanthanum nitrate was observed not only between testicle spermatogonia near seminiferous tubule wall and sertoli cells, but also between sertoli cells and primary spermatocyte or secondary spermatocyte. However, in control group lanthanum, nitrate was only found in the testicle sertoli cells between seminiferous tubule and near seminiferous tubule wall. CONCLUSION EMP exposure could increase the permeability of BTB in the mice.