The Gene Expression of Superoxide Dismutase and the changes of the Ultrastructure of Rat Testis After Exposed to Electromagnetics

摘要

Aims: To study the expression of superoxide dismutase (CuZnSOD, MnSOD) and the changes of ultrastructure in rat testis after electromagentic radiation(EMR) so as to investigate the mechanisms of EMR affect on procreation. Methods: As an in vivo model, male SD rats were used. Rats were exposured to the field of electromagentics. By reverse transcription-polymerase chain reaction (RT-PCR), manganese SOD (MnSOD) and copper/zinc SOD(CuZnSOD) mRNA in the testis have been tested and the testis samples were prepared for transmission electron microscopy to observe the ultrastructure at 6h, 12h, 24h and 48h after radiation. Results: In rat testis, MnSOD mRNA expression decreased at 24h and CuZnSOD mRNA expression didn't change after radiation, the ratio of MnSOD/CuZnSOD decreased at 24h after radiation. These were some degree injures in the testis at different time after radiation, the testis damage at 24h was serious than that at the other times after radiation. At 24h after exposured, the chromatin was gathered in the border and the endoplasmic reticulum (ER) was dilated and vacuolated in interstitial cell, spermatogonium cell of rat testis. The changes in sustentacular cell showed more serious than the other cells. Conclusion: The results showed that the MnSOD gene expression was decreased and the ultrastructure of rat testis were damaged after exposured to electromagnetics. These changes may induce the other effects, and the change of MnSOD expression might reflect a defensive mechanism of rat body against oxidative stress. If these changes wouldn't regain in time, it may cause the worse effects. Thus, EMR could induce some changes in the testis of rats so as to alter the procreative function.