In vivo study of structural integrity determination of antisense to NF-kB oligonucleotides in rat serum

摘要

Antisense is a promising drug which is considered as next generation drug due to its high specific nature to its target. Nonetheless it has a number of drawbacks, which, in fact, can be overcome by microencapsulating the drug with biodegradable and biocompatible polymer. However, characterization and quantisation of the encapsulated antisense is critical for determination of administered doses and for pharmacokinetic study. Specially, the drug integrity after administration is important to determine. Also for clinical use, the drug is radiated with gamma radiation, which might cause changes in the drug or polymer of the microsphere depending on the radiation intensity. Therefore, it is also required to determine the structural integrity of the irradiated microsphere. Previously we have demonstrated the integer form of gamma radiated and non-radiated microsphere in vitro (1). Here we have determined the structural integrity of the radiated and nonOradiated microsphere in vivo by HPLC.