Coherent Diffraction and Holographic Imaging of Individual Biomolecules Using Low-Energy Electrons

摘要

Modern microscopy techniques are aimed at imaging an individual molecule at atomic resolution. Here we show that low-energy electrons with kinetic energies of 50-250 eV offer a possibility of overcome the problem of radiation damage, and obtaining images of individual biomolecules. Two experimental schemes for obtaining images of individual molecules - holography and coherent diffraction imaging - are discussed and compared. Images of individual molecules obtained by both techniques, using low-energy electrons, are shown.