Molecular tagging of the thermosensitive genic male sterility gene in fine-grain indica rice

摘要

Molecular tagging of thermo-sensitive genic male sterility (TGMS) was attempted for the development of TGMS parental lines through marker-assisted rapid generation advancement. A stable TGMS donor, TS 29, was crossed with one finegrain popular variety, CO(R)49. The F2 population was raised during the winter season and the selfed seeds were harvested from 400 individual plants. DNA was extracted from all the plants. The stubbles were left and the sterile plants were identified. The F3 population was assayed for pollen viability and it ranged from 0 to 100%. DNA samples collected from the parents and extremely fertile and sterile individual progenies were identified. The corresponding F2 plants’DNA was used for carrying out bulk segregant analysis. One SSR marker, RM214, on chromosome 7 was putatively identified as being linked to the trait.