SINGLE-CELL LEVEL MEASUREMENT OF SHAPE, SHEAR STRESS DISTRIBUTION AND GENE EXPRESSION OF ENDOTHELIAL CELLS IN MICROFLUIDIC CHIPS

摘要

A measurement technique based on micro-PIV that allows a non-intrusive, non-tactile determination of the topography and shear stress distribution on endothelial cell cultures with a super-cell resolution is presented. The cells are grown in microfluidic perfusion chips where constant steady flow is applied. The expression of shear responsive gene KLF2 of individual cells is measured simultaneously with the shape and shear stress distribution of the same cell. Due to the super-cell resolution of the technique we can study the correlation of the measured quantities in different parts within the cell such as the nucleus or the cytoplasm. Results on human and chicken endothelial cells are shown.