Proliferation Effect of Low Dose Bisphenol A on Ventral Prostate and Relative Differential Gene Analysis in Adult Rats

摘要

The declining level of androgen during aging, associated with an inclining level of estrogen, has been hypothesized to be important in the development of benign prostatic hyperplasia. As an estrogenic endocrine disrupter, low dose bisphenol A (BPA) could induce hyperplasia prostate to proliferate and aggravate the symptom of BPH in male SD rats. We further hypothesized that low dose BPA could induce prostate to proliferate in adult rate. Male 3-monthold SD rats were treated with BPA (10, 30, or 90μg/kg, i. g. , daily), 17p-estradiol (E2, 10. 0μg/kg, s. c. , daily) , or vehicle for 4 weeks. We found that BPA (10μg/kg) significantly increased the height of ventral prostate(VP) epithelium (P<0.01) , while E2 significantly decreased the height of VP epithelium (P <0.01). BPA slightly increased serum E2 level, slightly decreased serum T level and significantly increased serum PSA level(P <0.01). E2 treatment also showed a obvious effect on hormone levels. Further microarray analysis and RT-PCR analysis results showed that BPA induced up-regulation of Ptgds and Fas, and downregulated expression of Pttgl in VP, especially up-regulated the gene Ptgds expression. We concluded that environment exposure to low dose of BPA may induce VP of adult rats to proliferate through up-regulating ptgds expression.