Voltammetric Approach for In-vivo Detecting Dopamine Level of Rat's Brain

摘要

Parkinson's disease (PD) is caused by insufficient release of dopamine (DA). Knowing the DA level of animal's brain could provide a direct evidence for evaluating novel treatments for PD, especially in the acute or long-term studies. In this study, we used nanogold particle surface modified platinum electrodes to measure DA using cyclic voltammetry and amperometry technique for in-vitro DA sensing. For in-vivo study, deep brain stimulation (DBS) was applied to increase DA release as a validation step for in-vivo DA detection using amperometry technique. For the future application of in-vivo DA sensing in freely moving animal, miniaturization of the system which can be incorporated to a radio frequency transmitter is essential. Our ultimate goal is to apply the wireless miniature DA sensing unit for in-vivo DA recording as a direct evident and evaluation tool for novel treatment such as repetitive transcranial magnetic stimulation (rTMS) treatment for PD animal study.